pST76A-SceP vector (V003036)

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LOCUS       V003036                 3810 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003036
VERSION     V003036
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Markerless gene replacement in Escherichia coli stimulated by a
            double-strand break in the chromosome
  JOURNAL   Nucleic Acids Res. 27 (22), 4409-4415 (1999)
   PUBMED   10536150
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center
            Hung. Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary
REFERENCE   3  (bases 1 to 3810)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3810)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1999"; volume: "27"; issue: "22"; pages:
            "4409-4415"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center Hung.
            Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..112
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             126..818
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             complement(979..1926)
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     rep_origin      complement(1974..2196)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(2821..3678)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3679..3783)
                     /label="AmpR promoter"