pST-2K vector (V003043)

LOCUS       40924_41275        4961 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Dual expression shuttle vector pST-2K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK.
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in mycobacteria
  JOURNAL   Appl. Environ. Microbiol. 79 (5), 1718-1729 (2013)
  PUBMED    23315736
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2012) Signal Transduction Lab-I, National 
            Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, Delhi 
            110067, India
REFERENCE   3  (bases 1 to 4961)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4961)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "5"; pages:
            "1718-1729"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2012) Signal Transduction Lab-I, National Institute of 
            Immunology, Aruna Asaf Ali Marg, New Delhi, Delhi 110067, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ClustalW v. 2.1
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..308
                     /note="Pmyc1tetO; UV15 promoter with tetO"
     protein_bind    179..196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    315..321
                     /label=SD sequence 1
                     /note="SD sequence 1"
     CDS             335..352
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      382..425
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      383..443
                     /label=transcription terminator 1
                     /note="transcription terminator 1"
                     /regulatory_class="terminator"
     regulatory      455..781
                     /label=UV15 promoter without tetO
                     /note="UV15 promoter without tetO"
                     /regulatory_class="promoter"
     misc_feature    784..790
                     /label=SD sequence 2
                     /note="SD sequence 2"
     misc_feature    791..820
                     /label=MCS2
                     /note="MCS2"
     CDS             821..844
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     terminator      863..905
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      934..980
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             1141..1953
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2288..2876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3043..4936
                     /label=oriM
                     /note="oriM"