pSSD3 vector (V003044)

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LOCUS       40924_41270        4234 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Unidentified cloning vector pSSD3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Kato S, Saeki M.
  TITLE     Signal sequence detection vector pSSD3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Kato S, Saeki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-1997) Contact:Seishi Kato Research Institute of 
            National Rehabilitation Center for the Disabled, Department of 
            Rehabilitation Engineering; 4-1 Namiki, Tokorozawa, Saitama 
            359-8555, Japan
REFERENCE   3  (bases 1 to 4234)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-1997) Contact:Seishi Kato Research Institute of National 
            Rehabilitation Center for the Disabled, Department of Rehabilitation
            Engineering; 4-1 Namiki, Tokorozawa, Saitama 359-8555, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          385..481
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     525..541
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        549..567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    581..586
                     /note="SmaI site for cloning of a target cDNA fragment
                     encoding a putative signal sequence"
     misc_feature    588..593
                     /note="PmaCI site for cloning of a target cDNA fragment
                     encoding a putative signal sequence"
     misc_feature    595..600
                     /note="EcoRV site for cloning of a target cDNA fragment
                     encoding a putative signal sequence"
     CDS             complement(1028..1039)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(1490..1508)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1510..1644)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1942..2530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2704..3561)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3562..3666)
                     /label=AmpR promoter
     rep_origin      complement(3693..4148)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4166..4182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"