pSS4332 vector (V003045)

Download GeneBank File(.gb)

LOCUS       V003045                 6668 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003045
VERSION     V003045
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Plaut RD, Stibitz S.
  TITLE     Improvements to a Markerless Allelic Exchange System for Bacillus
            anthracis
  JOURNAL   PLoS ONE 10 (12), E0142758 (2015)
   PUBMED   26624016
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Plaut RD, Stibitz S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2015) Center for Biologics Evaluation and
            Research, OVRR, DBPAP, Food and Drug Administration, 10903 New
            Hampshire Ave, Bldg 52/72, Rm 3310, Silver Spring, MD 20993, USA
REFERENCE   3  (bases 1 to 6668)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6668)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "12"; pages: "E0142758"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-SEP-2015) Center for Biologics Evaluation and Research, OVRR,
            DBPAP, Food and Drug Administration, 10903 New Hampshire Ave, Bldg
            52/72, Rm 3310, Silver Spring, MD 20993, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..708)
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             complement(767..1453)
                     /label="AmCyan"
                     /note="Anemonia majano cyan fluorescent protein"
     regulatory      complement(1480..1485)
                     /label="PFP1"
                     /note="PFP1"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(1503..1508)
                     /label="PFP1"
                     /note="PFP1"
                     /regulatory_class="minus_35_signal"
     CDS             complement(1629..2315)
                     /label="AmCyan"
                     /note="Anemonia majano cyan fluorescent protein"
     regulatory      complement(2342..2347)
                     /label="PFP1"
                     /note="PFP1"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(2365..2370)
                     /label="PFP1"
                     /note="PFP1"
                     /regulatory_class="minus_35_signal"
     CDS             complement(2380..3171)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3473..3491)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3512..3528)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3536..3552)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3560..3590)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3605..3626)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3914..4502)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     oriT            4840..4949
                     /label="oriT"
                     /note="incP origin of transfer"
     oriT            4955..5206
                     /note="from D2BpUC9Cam; tamdem repeat"
     oriT            5098..5207
                     /label="incP origin of transfer"
                     /note="incP origin of transfer"
     CDS             complement(5407..6408)
                     /label="repB"
                     /note="RepB replication protein"