Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V003048 | pSRE-Luc | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pSRE-Luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5743 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- Zheng C-F.
- Promoter:
- minP
pSRE-Luc vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pSRE-Luc vector Sequence
LOCUS 40924_41250 5743 bp DNA circular SYN 18-DEC-2018
DEFINITION Reporter vector pSRE-Luc, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5743)
AUTHORS Zheng C-F.
TITLE pSRE-Luc plasmid
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5743)
AUTHORS Zheng C-F.
TITLE Direct Submission
JOURNAL Submitted (12-MAR-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines, San Diego, CA 92037, USA
REFERENCE 3 (bases 1 to 5743)
AUTHORS Zheng C-F.
TITLE Direct Submission
JOURNAL Submitted (28-MAY-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines, San Diego, CA 92037, USA
REFERENCE 4 (bases 1 to 5743)
AUTHORS Zheng C-F.
TITLE Direct Submission
JOURNAL Submitted (01-FEB-1999) Technical Services, Stratagene, 11011 N.
Torrey Pines, San Diego, CA 92037, USA
REFERENCE 5 (bases 1 to 5743)
TITLE Direct Submission
REFERENCE 6 (bases 1 to 5743)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines,
San Diego, CA 92037, USA"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(28-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines,
San Diego, CA 92037, USA"
COMMENT SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
(01-FEB-1999) Technical Services, Stratagene, 11011 N. Torrey Pines,
San Diego, CA 92037, USA"
COMMENT SGRef: number: 5; type: "Journal Article"
COMMENT On Feb 1, 1999 this sequence version replaced gi:3165517.
FEATURES Location/Qualifiers
source 1..5743
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 32..136
/label=AmpR promoter
CDS 137..994
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1168..1755
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(1934..2074)
/label=bom
/note="basis of mobility region from pBR322"
polyA_signal 2313..2445
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 2539..2671
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2805..2836
/label=minP
/note="minimal TATA-box promoter with low basal activity"
CDS 2863..4512
/codon_start=1
/label=luciferase
/note="firefly luciferase"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 4646..4711
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 5281..5415
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"