pSRalphablastC vector (V003055)

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LOCUS       V003055                 5800 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003055
VERSION     V003055
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 5800)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5800)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard des
            Prairies, Laval, Quebec H7V 1B7, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    100..686
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1358
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             1431..2147
                     /label="ECFP"
                     /note="enhanced CFP"
     polyA_signal    complement(2173..2307)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(2316..2735)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(2755..2802)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     promoter        complement(2822..3151)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        3186..3290
                     /label="AmpR promoter"
     CDS             3291..4148
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4322..4910
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5172..5785
                     /label="SR-alpha promoter"
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human
                     T-lymphotrophic virus 1"