pSPV-EGFP vector (V003058)

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LOCUS       40924_41190        3457 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Poxvirus recombinant transfer vector pSPV-EGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Luo S, Peng Y, Diel DG, Rock DL.
  TITLE     Virus recombinant transfer vector pSPV-EGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Luo S, Peng Y, Diel DG, Rock DL.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2009) Pathobiology, University of Illinois at 
            Urbana-Champaign, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA
REFERENCE   3  (bases 1 to 3457)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2009) Pathobiology, University of Illinois at 
            Urbana-Champaign, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..33
                     /label=multiple cloning site 1 (MCS)
                     /note="multiple cloning site 1 (MCS)"
     regulatory      52..320
                     /label=early-late Vaccinia virus (VACV) VV7.5 promoter
                     /note="early-late Vaccinia virus (VACV) VV7.5 promoter"
                     /regulatory_class="promoter"
     CDS             340..1056
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1060..1085
                     /label=multiple cloning site 2 (MCS2)
                     /note="multiple cloning site 2 (MCS2)"
     promoter        complement(1095..1113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1371..1959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2133..2990)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2991..3095)
                     /label=AmpR promoter
     promoter        3441..3457
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"