pSPOM vector (V003060)

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LOCUS       V003060                 8294 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003060
VERSION     V003060
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Kawamura-Nagaya K, Ishibashi K, Huang YP, Miyashita S, Ishikawa M.
  TITLE     Replication protein of tobacco mosaic virus cotranslationally binds
            the 5' untranslated region of genomic RNA to enable viral
            replication
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 111 (16), E1620-E1628 (2014)
   PUBMED   24711385
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Ishikawa M, Kawamura K, Tsuda S, Meshi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2013) Contact:Masayuki Ishikawa National Institute
            of Agrobiological Sciences, Department of Plant Sciences,
            Plant-Microbe Interactions Research Unit; 2-1-2 Kan-non-dai,
            Tsukuba, Ibaraki 305-8602, Japan
REFERENCE   3  (bases 1 to 8294)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8294)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2014"; volume: "111"; issue: "16"; pages:
            "E1620-E1628"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2013) Contact:Masayuki Ishikawa National Institute of
            Agrobiological Sciences, Department of Plant Sciences, Plant-Microbe
            Interactions Research Unit; 2-1-2 Kan-non-dai, Tsukuba, Ibaraki
            305-8602, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..67
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             69..4919
                     /codon_start=1
                     /transl_except=(pos: 3417 .. 3419,  aa: Tyr)
                     /product="replication protein"
                     /label="replication protein"
                     /note="183-kDa (180K) protein; read through at 3417-3419"
                     /protein_id="BAO73175.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSYVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     YTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLDVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSRNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ*QLQIDSVFKGSNLFVAAPKTGDISDMQFYYDKCLPGNSTMMNNFDAVTM
                     RLTDISLNVKDCILDMSKSVAAPKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFN
                     APELSGIIDIENTASLVVDKFFDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQ
                     LADFDFVDLPAVDQYRHMIKAQPKQKLDTSIQTEYPALQTIVYHSKKINAIFGPLFSEL
                     TRQLLDSVDSSRFLFFTRKTPAQIEDFFGDLDSHVPMDILELDISKYDKSQNEFHCAVE
                     YEIWRRLGFEDFLGEVWKQGHRKTTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAAC
                     LASMLPMEKIIKGAFCGDDSLLYFPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRY
                     VIHHDRGCIVYYDPLKLISKLGAKHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAV
                     WEVHKTAPPGSFVYKSLVKYLSDKVLFRSLFIDGSSC"
     CDS             69..3419
                     /codon_start=1
                     /product="replication protein"
                     /label="replication protein"
                     /note="126-kDa (130K) protein"
                     /protein_id="BAO73176.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSYVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     YTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLDVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSRNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     CDS             4903..5706
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     OM). Accession#: P03582"
     CDS             5712..6188
                     /gene="CP"
                     /label="Capsid protein"
                     /note="Capsid protein from Tobacco mosaic virus (strain
                     OM). Accession#: P03571"
     misc_feature    6396..6419
                     /label="3' linker"
                     /note="3' linker"
     promoter        6523..6627
                     /label="AmpR promoter"
     CDS             6628..7485
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7659..8247
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    8263..8294
                     /label="5' linker and SP6 promoter"
                     /note="5' linker and SP6 promoter"