pSplPIGver2 vector (V003061)

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LOCUS       40924_41165        8867 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSplPIGver2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Gurskaia NG, Staroverov DB, Fradkov AF, Luk'ianov KA.
  TITLE     [Coding region of far-red fluorescent protein katushka contains a 
            strong donor splice site]
  JOURNAL   Bioorg. Khim. 37 (3), 425-428 (2011)
  PUBMED    21899059
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   Gurskaya NG, Staroverov DB, Zhang L, Fradkov AF, Markina NM, 
            Pereverzev AP, Lukyanov KA.
  TITLE     Analysis of alternative splicing of cassette exons at single-cell 
            level using two fluorescent proteins
  JOURNAL   Nucleic Acids Res. 40 (8), E57 (2012)
  PUBMED    22259036
REFERENCE   3  (bases 1 to 8867)
  AUTHORS   Gurskaya NG, Staroverov DB, Fradkov AF, Markina NM, Perverzev AP, 
            Lukyanov KA.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2015) Department of Genetics and Postgenomic 
            Technologies Laboratory of Biophotonics, M.M. Shemyakin and Yu.a. 
            Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy
            of Sciences, Ulitsa Miklukho-Maklaya, 16/10, Moscow 117997, Russian 
            Federation
REFERENCE   4  (bases 1 to 8867)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8867)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioorg. 
            Khim."; date: "2011"; volume: "37"; issue: "3"; pages: "425-428"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012"; volume: "40"; issue: "8"; pages: "E57"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2015) Department of Genetics and Postgenomic Technologies 
            Laboratory of Biophotonics, M.M. Shemyakin and Yu.a. Ovchinnikov 
            Institute of Bioorganic Chemistry of the Russian Academy of 
            Sciences, Ulitsa Miklukho-Maklaya, 16/10, Moscow 117997, Russian 
            Federation"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    590..612
                     /note="multiple cloning site; enhancer; TATA box"
     CDS             613..1317
                     /label=TurboFP635
                     /note="far-red-shifted derivative of red fluorescent
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
     misc_feature    1318..1333
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    4790..4816
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             4817..5530
                     /label=TagGFP2
                     /note="monomeric green fluorescent protein, also known as 
                     mTagGFP"
     polyA_signal    5655..5776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5783..6238)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6265..6369
                     /label=AmpR promoter
     promoter        6371..6728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6763..7554
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    7789..7836
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8165..8753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"