pSpcP-lac vector (V003075)

Download GeneBank File(.gb)

LOCUS       V003075                 4570 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003075
VERSION     V003075
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Chen Z, Zhang J, Huang D.
  TITLE     Escherichia coli-Pseudomonas shuttle plasmid pSpcP-lac based
            spectinomycin resistant gene and lacZ alpha gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Chen Z, Zhang J, Huang D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2002) Biotechnology, Institute of Plant
            Protection, 2, Yuan Ming Yuan Xi Lu, HaiDian District, Beijing,
            Beijing 100094, China
REFERENCE   3  (bases 1 to 4570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-JUN-2002) Biotechnology, Institute of Plant Protection, 2, Yuan
            Ming Yuan Xi Lu, HaiDian District, Beijing, Beijing 100094, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(244..832)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1128..1907
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     primer_bind     4046..4062
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4069..4087
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4096..4203
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(4216..4234)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4255..4271)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4279..4295)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4303..4333)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4348..4369)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."