pSpark V vector (V003076)

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LOCUS       40924_41052        3369 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSpark V, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Paz E.
  TITLE     pSpark V DNA Cloning Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Paz E.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2009) Molecular Biology, Canvax Biotech S.L., 
            Estocolmo, Cordoba, Cordoba 14014, Spain
REFERENCE   3  (bases 1 to 3369)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-AUG-2009) Molecular Biology, Canvax Biotech S.L., Estocolmo, 
            Cordoba, Cordoba 14014, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             109..297
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    402..546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(732..1320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1494..2351)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2352..2456)
                     /label=AmpR promoter
     rep_origin      complement(2534..2989)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3130..3146
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3153..3171
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3307..3325)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3343..3359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"