pSP64bb2sp4 vector (V003081)

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LOCUS       40924_41017        3632 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Microarray spiking control vector pSP64bb2sp4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Sato M, Mitra RM, Coller J, Wang D, Spivey NW, Dewdney J, Denoux C, 
            Glazebrook J, Katagiri F.
  TITLE     A high-performance, small-scale microarray for expression profiling 
            of many samples in Arabidopsis-pathogen studies
  JOURNAL   Plant J. 49 (3), 565-577 (2007)
  PUBMED    17181774
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Sato M, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2006) Department of Plant Biology, University of 
            Minnesota, 1500 Gortner Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 3632)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "49"; issue: "3"; pages: "565-577"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2006) Department of Plant Biology, University of Minnesota, 
            1500 Gortner Avenue, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..688
                     /label=transcribed region
                     /note="transcribed region"
     misc_feature    1..579
                     /label=backbone sequence 2 (bb2)
                     /note="backbone sequence 2 (bb2)"
     misc_feature    580..647
                     /label=spiking control 3 (sp3)
                     /note="spiking control 3 (sp3)"
     primer_bind     complement(702..718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(726..742)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(750..780)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(795..816)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1104..1692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1866..2723)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2724..2828)
                     /label=AmpR promoter
     promoter        3616..3632
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"