pSOS-X vector (V003091)

LOCUS       40924_40962        4701 bp DNA     circular SYN 18-DEC-2018
DEFINITION  ORF selection and expression vector pSOS-X, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Cox JC, Lape J, Sayed MA, Hellinga HW.
  TITLE     Protein fabrication automation
  JOURNAL   Protein Sci. 16 (3), 379-390 (2007)
  PUBMED    17242375
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Cox JC, Hellinga HW.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Biochemistry, Duke University Medical 
            Center, Research Drive, DUMC 3711, Nanaline 413, Durham, NC 27710, 
            USA
REFERENCE   3  (bases 1 to 4701)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4701)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2007"; volume: "16"; issue: "3"; pages: "379-390"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2006) Biochemistry, Duke University Medical Center, Research
            Drive, DUMC 3711, Nanaline 413, Durham, NC 27710, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(240..828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1002..1859)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1860..1964)
                     /label=AmpR promoter
     regulatory      2106..2151
                     /gene="lacI"
                     /regulatory_class="promoter"
     CDS             2172..3251
                     /label=lacI
                     /note="lac repressor"
     protein_bind    3267..3288
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3503..3531
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3539..3555
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      3611..3616
                     /label=Shine-Delgarno sequence
                     /note="Shine-Delgarno sequence"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    3617..3624
                     /label=PacI restriction site
                     /note="PacI restriction site"
     misc_feature    3633..3638
                     /label=NaeI restriction site
                     /note="NaeI restriction site"
     CDS             3639..3656
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    3657..3659
                     /label=amber stop codon
                     /note="amber stop codon"
     CDS             3666..4319
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      4492..4578
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4670..4697
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"