pSOS plasmid pSOS vector (V003092)

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LOCUS       40924_40957        4208 bp DNA     circular SYN 18-DEC-2018
DEFINITION  ORF selection vector pSOS plasmid pSOS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Cox JC, Lape J, Sayed MA, Hellinga HW.
  TITLE     Protein fabrication automation
  JOURNAL   Protein Sci. 16 (3), 379-390 (2007)
  PUBMED    17242375
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Cox JC, Hellinga HW.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Biochemistry, Duke University Medical 
            Center, Research Drive, DUMC 3711, Nanaline 413, Durham, NC 27710, 
            USA
REFERENCE   3  (bases 1 to 4208)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4208)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2007"; volume: "16"; issue: "3"; pages: "379-390"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2006) Biochemistry, Duke University Medical Center, Research
            Drive, DUMC 3711, Nanaline 413, Durham, NC 27710, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      70..75
                     /label=Shine-Delgarno sequence
                     /note="Shine-Delgarno sequence"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    76..83
                     /label=PacI restriction site
                     /note="PacI restriction site"
     misc_feature    92..97
                     /label=HpaI restriction site
                     /note="HpaI restriction site"
     CDS             116..769
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(846..864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(871..887)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1025..1324
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      1538..1675
                     /gene="aphA1"
                     /regulatory_class="promoter"
     CDS             1676..2467
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2677..3048
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      3189..3777
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4065..4086
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4101..4131
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4139..4155
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4163..4179
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"