pSolycp00014 vector (V003111)

LOCUS       40924_40862       11778 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSolycp00014 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Aoki K, Kurabayashi A, Suda K, Odahara M, Goto DB, Shibata D.
  TITLE     5'-flanking region of Solyc06g083030 fused to SlHTA6-EYFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   Aoki K, Kurabayashi A, Suda K, Odahara M, Goto DB, Shibata D.
  TITLE     Tomato HTA6 fused to EYFP driven by 35S omega promoter
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11778)
  AUTHORS   Aoki K, Kurabayashi A, Suda K, Odahara M, Goto D, Shibata D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2011) Contact:Koh Aoki Osaka Prefecture 
            University, Department of Life and Environmental Sciences; Naka-ku, 
            Gakuen-cho, 1-1, Sakai, Osaka 599-8531, Japan E-mail 
            :kaoki@plant.osakafu-u.ac.jp Phone :81-72-254-9421 Fax 
            :81-72-254-9918
REFERENCE   4  (bases 1 to 11778)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11778)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (24-NOV-2011) Contact:Koh Aoki Osaka Prefecture University, 
            Department of Life and Environmental Sciences"; volume: " Naka-ku, 
            Gakuen-cho, 1-1, Sakai, Osaka 599-8531, Japan E-mail 
            :kaoki@plant.osakafu-u.ac.jp Phone :81-72-254-9421 Fax "; pages: 
            "81-72-254-991"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(21..45)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    345..369
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      390..1855
                     /note="tomato promoter, BLAST search(vs SL2.40.fasta) 
                     result/SL2.40ch06:44882897..44881431"
                     /regulatory_class="promoter"
     protein_bind    complement(1872..1896)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1921..2358
                     /gene="SlHTA6"
                     /note="The nucleotide sequence is identical to GenBank 
                     JN790634.1.; putative histone 2A protein"
     gene            1921..2358
                     /gene="SlHTA6"
                     /label=SlHTA6
     CDS             2365..3081
                     /label=EYFP
                     /note="enhanced YFP"
     terminator      3101..3348
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3370..3386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3394..3410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3418..3448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3463..3484)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3675..4352
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             4421..5209
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5269..5443
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(5521..5545)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5970..6761
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6851..7439
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7625..7765)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8109..8303)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8372..9436)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9873..10499)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"