pSMB47 for Tn5251 and Tn916 vector (V003133)

LOCUS       V003133                 8774 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003133
VERSION     V003133
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Brehm J, Salmond G, Minton N.
  TITLE     Sequence of the adenine methylase gene of the Streptococcus faecalis
            plasmid pAM beta 1
  JOURNAL   Nucleic Acids Res. 15 (7), 3177 (1987)
   PUBMED   3104884
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Rose RE.
  TITLE     The nucleotide sequence of pACYC184
  JOURNAL   Nucleic Acids Res. 16 (1), 355 (1988)
   PUBMED   3340533
REFERENCE   3  (bases 1025 to 1263)
  AUTHORS   Kilic AO, Vijayakumar MN, al-Khaldi SF.
  TITLE     Identification and nucleotide sequence analysis of a
            transfer-related region in the streptococcal conjugative transposon
            Tn5252
  JOURNAL   J. Bacteriol. 176 (16), 5145-5150 (1994)
   PUBMED   8051031
REFERENCE   4  (bases 1 to 8774)
  AUTHORS   Provvedi R, Manganelli R, Pozzi G.
  TITLE     Characterization of conjugative transposon Tn5251 of Streptococcus
            pneumoniae
  JOURNAL   FEMS Microbiol. Lett. 135 (2-3), 231-236 (1996)
   PUBMED   8595862
REFERENCE   5  (bases 1 to 8774)
  AUTHORS   Manganelli R, Provvedi R, Berneri C, Oggioni MR, Pozzi G.
  TITLE     Insertion vectors for in vivo construction of recombinant
            conjugative transposons
  JOURNAL   Unpublished
REFERENCE   6  (bases 1 to 8774)
  AUTHORS   Manganelli R, Provvedi R, Berneri C, Oggioni MR, Pozzi G.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-1996) Microbiology, University of Siena, Via
            Laterina 8, Siena, Si 53100, Italy
REFERENCE   7  (bases 1 to 8774)
  TITLE     Direct Submission
REFERENCE   8  (bases 1 to 8774)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1987"; volume: "15"; issue: "7"; pages: "3177"
            SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1988"; volume: "16"; issue: "1"; pages: "355"
            SGRef: number: 3; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1994"; volume: "176"; issue: "16"; pages:
            "5145-5150"
            SGRef: number: 4; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett. 135 (2-3), 231-236 (1996)"
            SGRef: number: 5; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 6; type: "Journal Article"; journalName: "Submitted
            (04-SEP-1996) Microbiology, University of Siena, Via Laterina 8,
            Siena, Si 53100, Italy"
            SGRef: number: 7; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1028
                     /note="similar to Enterococcus faecalis Tn916 of GenBank
                     Accession Number U09422"
                     /citation=[4]
     misc_feature    1025..1263
                     /note="similar to S. pneumoniae Tn5252 of GenBank Accession
                     Number L29324"
                     /citation=[3]
     CDS             complement(1625..2281)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2282..2384)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2910..3455)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3717..4451)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     misc_feature    4459..5370
                     /note="similar to S. agalactiae pIP501 of GenBank Accession
                     Number U00453"
     CDS             5422..6609
                     /label="TcR"
                     /note="tetracycline efflux protein"
     misc_feature    7423..8773
                     /note="similar to S. pneumoniae tet(M) gene of GenBank
                     Accession Number X90939"
                     /citation=[4]