pSlip7 vector (V003146)

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LOCUS       40924_40652        4431 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSlip7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Qiu F, Guo L, Wen TJ, Liu F, Ashlock DA, Schnable PS.
  TITLE     DNA sequence-based 'bar codes' for tracking the origins of expressed
            sequence tags from a maize cDNA library constructed using multiple 
            mRNA sources
  JOURNAL   Plant Physiol. 133 (2), 475-481 (2003)
  PUBMED    14555776
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Liu F, Schnable PS.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2003) Iowa State University, B420 Agronomy Hall, 
            Ames, IA 50010, USA
REFERENCE   3  (bases 1 to 4431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2003"; volume: "133"; issue: "2"; pages: "475-481"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2003) Iowa State University, B420 Agronomy Hall, Ames, IA 
            50010, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..458
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             746..1603
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     protein_bind    1708..1741
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      1819..2407
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2685..2762
                     /label=lacI promoter
     protein_bind    3857..3878
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3893..3923
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3931..3947
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3979..3997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4096..4113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4115..4132
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4134..4151
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        complement(4251..4269)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4277..4293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"