pSLIE1-3_Mos1mariner_ORF vector (V003147)

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LOCUS       V003147                 5920 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003147
VERSION     V003147
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Alrefaei YN, Suttiprapa S, Okatcha TI, Rinaldi G, Brindley PJ.
  TITLE     A plasmid encoding the Mos-1 mariner transposase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Alrefaei YN, Suttiprapa S, Okatcha TI, Rinaldi G, Brindley PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2011) Microbiology, Immunology and Tropical
            Medicine, George Washington University, 2300 Eye Street, NW,
            Washington, DC 20037, USA
REFERENCE   3  (bases 1 to 5920)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-SEP-2011) Microbiology, Immunology and Tropical Medicine, George
            Washington University, 2300 Eye Street, NW, Washington, DC 20037,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..485
                     /label="hr5 enhancer"
                     /note="baculovirus early transcription enhancer"
     promoter        489..1080
                     /label="IE1 promoter"
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     CDS             1107..2141
                     /gene="marinerT"
                     /label="Mariner Mos1 transposase"
                     /note="Mariner Mos1 transposase from Drosophila mauritiana.
                     Accession#: Q7JQ07"
     terminator      2652..2959
                     /label="IE1 terminator"
                     /note="terminator of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        3735..3839
                     /label="AmpR promoter"
     CDS             3840..4697
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4871..5459
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5747..5768
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5783..5813
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5821..5837
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5845..5861
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"