pSK9 vector (V003155)

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LOCUS       40924_40582        6267 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSK9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Kuchmina E, Wallner T, Kryazhov S, Zinchenko VV, Wilde A.
  TITLE     An expression system for regulated protein production in 
            Synechocystis sp. PCC 6803 and its application for construction of a
            conditional knockout of the ferrochelatase enzyme
  JOURNAL   J. Biotechnol. 162 (1), 75-80 (2012)
  PUBMED    22749908
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Zinchenko VV, Kryazhov SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2011) Dept. of Genetics, Biology Division, Moscow 
            State University, Moscow 119899, Russia
REFERENCE   3  (bases 1 to 6267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2012"; volume: "162"; issue: "1"; pages: 
            "75-80"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-DEC-2011) Dept. of Genetics, Biology Division, Moscow State 
            University, Moscow 119899, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    656..1145
                     /note="p6803-platform; derived from Synechocystis sp. PCC
                     6803 chromosome in INSD: BA000022"
     CDS             complement(1819..2475)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    2581..3058
                     /note="leader region of petJ gene derived from
                     Synechocystis sp. PCC 6803 chromosome in INSD: BA000022"
     misc_feature    3059..4063
                     /note="p6803-platform; derived from Synechocystis sp. PCC
                     6803 chromosome in INSD: BA000022"
     promoter        complement(4082..4100)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4121..4137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4145..4161)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4169..4199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4214..4235)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4523..5111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5285..6142)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6143..6247)
                     /label=AmpR promoter