pSK(+)PICT1 vector (V003167)

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LOCUS       40924_40517        5607 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pSK(+)PICT1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 5607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva RbcS promoter
                     /note="Ulva RbcS promoter"
                     /regulatory_class="promoter"
     repeat_region   558..668
                     /label=derived from Escherichia coli transposon Tn5
                     /note="derived from Escherichia coli transposon Tn5"
     intron          680..842
                     /number=1
                     /note="derived from Ulva RbcS"
     CDS             complement(990..1646)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1647..1749)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     intron          1843..2175
                     /number=1
                     /note="derived from Ulva RbcS"
     repeat_region   2182..2293
                     /label=derived from Escherichia coli transposon Tn5
                     /note="derived from Escherichia coli transposon Tn5"
     regulatory      2301..2689
                     /label=Ulva RbcS terminator
                     /note="Ulva RbcS terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2705..2721)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2747..2765)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2772..2788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2933..3388
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3417..3521
                     /label=AmpR promoter
     CDS             3522..4379
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4553..5141
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5429..5450
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5465..5495
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5503..5519
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5527..5543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5564..5582
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"