pP02218-nat-cel8A vector (V004191)

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LOCUS       V004191                 6904 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004191
VERSION     V004191
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Heinze S, Zimmermann K, Ludwig C, Heinzlmeir S, Schwarz WH, Zverlov
            VV, Liebl W, Kornberger P.
  TITLE     Efficient secretory heterologous enzyme production in the novel host
            Paenibacillus polymyxa by usage of its promoter sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Heinze S, Zimmermann K, Ludwig C, Heinzlmeir S, Schwarz WH, Zverlov
            VV, Liebl W, Kornberger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Department for Microbiology, Technical
            University of Munich, Emil-Ramann-Str. 4, Freising, Bavaria 85354,
            Germany
REFERENCE   3  (bases 1 to 6904)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6904)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAY-2018) Department for Microbiology, Technical University of
            Munich, Emil-Ramann-Str. 4, Freising, Bavaria 85354, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(79..667)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(841..1698)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1699..1803)
                     /label="AmpR promoter"
     CDS             complement(1832..2116)
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label="traJ"
                     /protein_id="AXF54530.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGQQKAHYVNHHPNQVFWGRGAVKH"
     gene            complement(1832..2116)
                     /gene="traJ"
                     /label="traJ"
     oriT            complement(2149..2258)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(2515..3276)
                     /codon_start=1
                     /gene="kanR"
                     /product="KanR"
                     /label="kanR"
                     /note="Kanamycin Resistance"
                     /protein_id="AXF54531.1"
                     /translation="MNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFF
                     SILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEK
                     LLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            complement(2515..3276)
                     /gene="kanR"
                     /label="kanR"
     CDS             complement(3448..4449)
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      4866..5277
                     /label="P02218 promoter from P. polymyxa DSM292"
                     /note="P02218 promoter from P. polymyxa DSM292"
                     /regulatory_class="promoter"
     CDS             5308..6717
                     /gene="celA"
                     /label="Endoglucanase A"
                     /note="Endoglucanase A from Acetivibrio thermocellus
                     (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 /
                     NCIMB 10682 / NRRL B-4536 / VPI 7372). Accession#: A3DC29"
     CDS             6724..6741
                     /label="6xHis"
                     /note="6xHis affinity tag"