Basic Vector Information
- Vector Name:
- pORI101
- Length:
- 5316 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden PD.
pORI101 vector Map
pORI101 vector Sequence
LOCUS V004211 5316 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V004211 VERSION V004211 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 5316) AUTHORS Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden PD. TITLE Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon JOURNAL Tuberculosis (Edinb) 87 (6), 481-488 (2007) PUBMED 17888739 REFERENCE 2 (bases 1 to 5316) AUTHORS Bourn WR. TITLE Direct Submission JOURNAL Submitted (10-JAN-2007) Medical Virology and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road Observatory, Cape Town 7925, South Africa REFERENCE 3 (bases 1 to 5316) TITLE Direct Submission REFERENCE 4 (bases 1 to 5316) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Tuberculosis (Edinb)"; date: "2007"; volume: "87"; issue: "6"; pages: "481-488" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (10-JAN-2007) Medical Virology and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road Observatory, Cape Town 7925, South Africa" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5316 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 250..838 /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 1126..1147 /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 1162..1192 /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 1200..1216 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 1224..1240 /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1328..1344) /label="KS primer" /note="common sequencing primer, one of multiple similar variants" rep_origin 1374..3961 /note="mycobacterial origin; OriM" CDS complement(4202..5197) /gene="hyg" /label="Hygromycin-B 7''-O-kinase" /note="Hygromycin-B 7''-O-kinase from Streptomyces hygroscopicus. Accession#: P09979"
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