pOpt_Clover_Hyg vector (V004221)

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LOCUS       V004221                 6831 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004221
VERSION     V004221
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Lauersen KJ, Mussgnug JH, Kruse O.
  TITLE     Efficient targeted expression of nuclear transgenes in Chlamydomonas
            reinhardtii with a novel, modular vector toolkit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Lauersen KJ, Mussgnug JH, Kruse O.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2014) Algae Biotechnology and Bioenergy,
            University of Bielefeld Center for Biotechnology, Universitaetsstr
            27, Bielefeld, NRW 33615, Germany
REFERENCE   3  (bases 1 to 6831)
  AUTHORS   Lauersen KJ, Mussgnug JH, Kruse O.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Algae Biotechnology and Bioenergy,
            University of Bielefeld Center for Biotechnology, Universitaetsstr
            27, Bielefeld, NRW 33615, Germany
REFERENCE   4  (bases 1 to 6831)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-JUN-2014) Algae Biotechnology and Bioenergy, University of
            Bielefeld Center for Biotechnology, Universitaetsstr 27, Bielefeld,
            NRW 33615, Germany"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2016) Algae Biotechnology and Bioenergy, University of
            Bielefeld Center for Biotechnology, Universitaetsstr 27, Bielefeld,
            NRW 33615, Germany"
            SGRef: number: 4; type: "Journal Article"
            On May 18, 2016 this sequence version replaced KM061067.1.
FEATURES             Location/Qualifiers
     source          1..6831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     489..505
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        515..533
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1247..1258
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             2269..2280
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             2293..2316
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             3266..4261
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     promoter        complement(4531..4549)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4570..4586)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4594..4610)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4618..4648)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4663..4684)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4972..5560)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5734..6591)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6592..6696)
                     /label="AmpR promoter"
     rep_origin      complement(join(6723..6831,1..347))
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"