pNV18 vector (V004250)

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LOCUS       40924_33652        4411 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pNV18 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Chiba K, Hoshino Y, Ishino K, Kogure T, Mikami Y, Uehara Y, Ishikawa
            J.
  TITLE     Construction of a pair of practical Nocardia-Escherichia coli 
            shuttle vectors
  JOURNAL   Jpn. J. Infect. Dis. 60 (1), 45-47 (2007)
  PUBMED    17314425
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Ishikawa J, Chiba K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2006) Contact:Jun Ishikawa National Institute of 
            Infectious Diseases, Department of Bioactive Molecules; 1-23-1 
            Toyama, Shinjuku, Tokyo 162-8640, Japan URL 
            :http://nocardia.nih.go.jp/
REFERENCE   3  (bases 1 to 4411)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Jpn. J. 
            Infect. Dis."; date: "2007"; volume: "60"; issue: "1"; pages: 
            "45-47"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JUL-2006) Contact:Jun Ishikawa National Institute of Infectious 
            Diseases, Department of Bioactive Molecules; 1-23-1 Toyama, 
            Shinjuku, Tokyo 162-8640, Japan URL :http://nocardia.nih.go.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Dec 18, 2007 this sequence version replaced AB267085.1.
FEATURES             Location/Qualifiers
     source          1..4411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(172..228)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(238..254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(262..278)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(286..316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(331..352)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(640..1228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1515..2306)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    2662..4411
                     /label=derived from pAL5000
                     /note="derived from pAL5000"
     CDS             complement(2746..3093)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein RepB"
                     /label=repB
                     /protein_id="BAF02356.2"
                     /translation="MSDGYSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQER
                     SEWLAEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAAQK
                     AHNEADNPPLF"
     gene            complement(2746..3093)
                     /gene="repB"
                     /label=repB
     CDS             complement(3090..4016)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein RepA"
                     /label=repA
                     /protein_id="BAF02357.1"
                     /translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEANP
                     TALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEYAR
                     RKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAELGAN
                     MPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAEC
                     HARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAIS
                     RKGAAARTAASTVARRAKSASAMEALL"
     gene            complement(3090..4016)
                     /gene="repA"
                     /label=repA