Basic Vector Information
- Vector Name:
- pNUversa
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10873 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Sakai F, Sonaty G, Watson D, Klugman KP, Vidal JE.
- Promoter:
- tac
pNUversa vector Map
pNUversa vector Sequence
LOCUS 40924_33647 10873 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNUversa, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10873)
AUTHORS Sakai F, Sonaty G, Watson D, Klugman KP, Vidal JE.
TITLE Development and characterization of a synthetic DNA, NUversa, to be
used as a standard in quantitative polymerase chain reactions for
molecular pneumococcal serotyping
JOURNAL FEMS Microbiol. Lett. 364 (17) (2017)
PUBMED 28903467
REFERENCE 2 (bases 1 to 10873)
AUTHORS Sakai F, Vidal JE.
TITLE Direct Submission
JOURNAL Submitted (17-JUL-2017) Hubert Department of Global Health, Rollins
School of Public Health, Emory University, 1518 Clifton Rd.,
Atlanta, GA 30329, USA
REFERENCE 3 (bases 1 to 10873)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10873)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
Microbiol. Lett. 364 (17) (2017)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-JUL-2017) Hubert Department of Global Health, Rollins School of
Public Health, Emory University, 1518 Clifton Rd., Atlanta, GA
30329, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10873
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 396..424
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 432..448
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 461..535
/note="real time PCR target sequence - Streptococcus
pneumoniae lytA gene fragment"
misc_feature 536..696
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 1 cps gene fragment"
misc_feature 697..807
/note="real time PCR target sequence -Streptococcus
pneumoniae serotype 2 cps gene fragment"
misc_feature 808..892
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 3 cps gene fragment"
misc_feature 893..1106
/note="real time PCR target sequence -Streptococcus
pneumoniae serotype 4 cps gene fragment"
misc_feature 1107..1188
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 5 cps gene fragment"
misc_feature 1189..1316
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 6ABCD cps gene fragment"
misc_feature 1317..1465
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 6CD cps gene fragment"
misc_feature 1466..1569
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 7AF cps gene fragment"
misc_feature 1570..1716
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 7BC/40 cps gene fragment"
misc_feature 1717..1848
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 7C cps gene fragment"
misc_feature 1849..1931
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 8 cps gene fragment"
misc_feature 1932..2085
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 9VA cps gene fragment"
misc_feature 2086..2200
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 9LN cps gene fragment"
misc_feature 2201..2337
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 10A cps gene fragment"
misc_feature 2338..2453
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 10B cps gene fragment"
misc_feature 2454..2590
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 10CF cps gene fragment"
misc_feature 2591..2697
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 11AD cps gene fragment"
misc_feature 2698..2826
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 11BC cps gene fragment"
misc_feature 2827..2919
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 11F cps gene fragment"
misc_feature 2920..2999
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 12ABF/44/46 cps gene fragment"
misc_feature 3000..3135
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 13 cps gene fragment"
misc_feature 3136..3223
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 14 cps gene fragment"
misc_feature 3224..3353
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 15AF cps gene fragment"
misc_feature 3354..3446
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 15ABCF cps gene fragment"
misc_feature 3447..3578
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 16A cps gene fragment"
misc_feature 3579..3777
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 16F cps gene fragment"
misc_feature 3778..3889
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 17A cps gene fragment"
misc_feature 3890..4019
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 17F cps gene fragment"
misc_feature 4020..4167
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 18ABCF cps gene fragment"
misc_feature 4168..4256
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 19A cps gene fragment"
misc_feature 4257..4331
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 19BF cps gene fragment"
misc_feature 4332..4552
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 19F cps gene fragment"
misc_feature 4553..4658
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 19'F' cps gene fragment"
misc_feature 4659..4773
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 19C cps gene fragment"
misc_feature 4774..4865
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 20 cps gene fragment"
misc_feature 4866..4952
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 21 cps gene fragment"
misc_feature 4953..5041
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 22AF cps gene fragment"
misc_feature 5042..5127
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 23A cps gene fragment"
misc_feature 5128..5205
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 23B cps gene fragment"
misc_feature 5206..5431
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 23F cps gene fragment"
misc_feature 5432..5551
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 24A cps gene fragment"
misc_feature 5552..5684
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 24BF cps gene fragment"
misc_feature 5685..5812
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 25AF cps gene fragment"
misc_feature 5813..5891
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 27 cps gene fragment"
misc_feature 5892..6032
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 28AF cps gene fragment"
misc_feature 6033..6113
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 29 cps gene fragment"
misc_feature 6114..6259
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 31 cps gene fragment"
misc_feature 6260..6393
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 32AF cps gene fragment"
misc_feature 6394..6498
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 33AF/37 cps gene fragment"
misc_feature 6499..6644
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 33B cps gene fragment"
misc_feature 6645..6791
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 33C cps gene fragment"
misc_feature 6792..6872
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 33D cps gene fragment"
misc_feature 6873..7016
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 34 cps gene fragment"
misc_feature 7017..7124
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 35AC/42 cps gene fragment"
misc_feature 7125..7248
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 35B cps gene fragment"
misc_feature 7249..7393
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 35F/47F cps gene fragment"
misc_feature 7394..7541
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 36 cps gene fragment"
misc_feature 7542..7619
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 38 cps gene fragment"
misc_feature 7620..7762
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 39 cps gene fragment"
misc_feature 7763..7876
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 41A cps gene fragment"
misc_feature 7877..8010
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 41F cps gene fragment"
misc_feature 8011..8160
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 43 cps gene fragment"
misc_feature 8161..8248
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 45 cps gene fragment"
misc_feature 8249..8381
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 46 cps gene fragment"
misc_feature 8382..8502
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 47AF cps gene fragment"
misc_feature 8503..8635
/note="real time PCR target sequence - Streptococcus
pneumoniae serotype 48 cps gene fragment"
primer_bind complement(8652..8668)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 8676..8692
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8700..8730)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(8745..8766)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9054..9642)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9816..10673)
/label=AmpR
/note="beta-lactamase"
promoter complement(10674..10778)
/label=AmpR promoter
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