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pNUversa vector (V004251)

Basic Vector Information

Vector Name:
pNUversa
Antibiotic Resistance:
Ampicillin
Length:
10873 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Sakai F, Sonaty G, Watson D, Klugman KP, Vidal JE.
Promoter:
tac

pNUversa vector Vector Map

pNUversa10873 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500M13 fwdtac promoterlac operatorreal time PCR target sequence - Streptococcus pneumoniae lytA gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 1 cps gene fragmentreal time PCR target sequence -Streptococcus pneumoniae serotype 2 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 3 cps gene fragmentreal time PCR target sequence -Streptococcus pneumoniae serotype 4 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 5 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 6ABCD cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 6CD cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 7AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 7BC/40 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 7C cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 8 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 9VA cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 9LN cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 10A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 10B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 10CF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 11AD cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 11BC cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 11F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 12ABF/44/46 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 13 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 14 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 15AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 15ABCF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 16A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 16F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 17A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 17F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 18ABCF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19BF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19'F' cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19C cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 20 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 21 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 22AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 23A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 23B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 23F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 24A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 24BF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 25AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 27 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 28AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 29 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 31 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 32AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33AF/37 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33C cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33D cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 34 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 35AC/42 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 35B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 35F/47F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 36 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 38 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 39 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 41A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 41F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 43 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 45 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 46 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 47AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 48 cps gene fragmentM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

pNUversa vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33647       10873 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNUversa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Sakai F, Sonaty G, Watson D, Klugman KP, Vidal JE.
  TITLE     Development and characterization of a synthetic DNA, NUversa, to be 
            used as a standard in quantitative polymerase chain reactions for 
            molecular pneumococcal serotyping
  JOURNAL   FEMS Microbiol. Lett. 364 (17) (2017)
  PUBMED    28903467
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   Sakai F, Vidal JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2017) Hubert Department of Global Health, Rollins 
            School of Public Health, Emory University, 1518 Clifton Rd., 
            Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 10873)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10873)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett. 364 (17) (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUL-2017) Hubert Department of Global Health, Rollins School of 
            Public Health, Emory University, 1518 Clifton Rd., Atlanta, GA 
            30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        396..424
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    432..448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    461..535
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae lytA gene fragment"
     misc_feature    536..696
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 1 cps gene fragment"
     misc_feature    697..807
                     /note="real time PCR target sequence -Streptococcus
                     pneumoniae serotype 2 cps gene fragment"
     misc_feature    808..892
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 3 cps gene fragment"
     misc_feature    893..1106
                     /note="real time PCR target sequence -Streptococcus
                     pneumoniae serotype 4 cps gene fragment"
     misc_feature    1107..1188
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 5 cps gene fragment"
     misc_feature    1189..1316
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 6ABCD cps gene fragment"
     misc_feature    1317..1465
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 6CD cps gene fragment"
     misc_feature    1466..1569
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7AF cps gene fragment"
     misc_feature    1570..1716
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7BC/40 cps gene fragment"
     misc_feature    1717..1848
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7C cps gene fragment"
     misc_feature    1849..1931
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 8 cps gene fragment"
     misc_feature    1932..2085
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 9VA cps gene fragment"
     misc_feature    2086..2200
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 9LN cps gene fragment"
     misc_feature    2201..2337
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10A cps gene fragment"
     misc_feature    2338..2453
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10B cps gene fragment"
     misc_feature    2454..2590
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10CF cps gene fragment"
     misc_feature    2591..2697
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11AD cps gene fragment"
     misc_feature    2698..2826
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11BC cps gene fragment"
     misc_feature    2827..2919
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11F cps gene fragment"
     misc_feature    2920..2999
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 12ABF/44/46 cps gene fragment"
     misc_feature    3000..3135
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 13 cps gene fragment"
     misc_feature    3136..3223
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 14 cps gene fragment"
     misc_feature    3224..3353
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 15AF cps gene fragment"
     misc_feature    3354..3446
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 15ABCF cps gene fragment"
     misc_feature    3447..3578
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 16A cps gene fragment"
     misc_feature    3579..3777
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 16F cps gene fragment"
     misc_feature    3778..3889
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 17A cps gene fragment"
     misc_feature    3890..4019
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 17F cps gene fragment"
     misc_feature    4020..4167
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 18ABCF cps gene fragment"
     misc_feature    4168..4256
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19A cps gene fragment"
     misc_feature    4257..4331
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19BF cps gene fragment"
     misc_feature    4332..4552
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19F cps gene fragment"
     misc_feature    4553..4658
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19'F' cps gene fragment"
     misc_feature    4659..4773
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19C cps gene fragment"
     misc_feature    4774..4865
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 20 cps gene fragment"
     misc_feature    4866..4952
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 21 cps gene fragment"
     misc_feature    4953..5041
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 22AF cps gene fragment"
     misc_feature    5042..5127
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23A cps gene fragment"
     misc_feature    5128..5205
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23B cps gene fragment"
     misc_feature    5206..5431
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23F cps gene fragment"
     misc_feature    5432..5551
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 24A cps gene fragment"
     misc_feature    5552..5684
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 24BF cps gene fragment"
     misc_feature    5685..5812
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 25AF cps gene fragment"
     misc_feature    5813..5891
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 27 cps gene fragment"
     misc_feature    5892..6032
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 28AF cps gene fragment"
     misc_feature    6033..6113
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 29 cps gene fragment"
     misc_feature    6114..6259
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 31 cps gene fragment"
     misc_feature    6260..6393
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 32AF cps gene fragment"
     misc_feature    6394..6498
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33AF/37 cps gene fragment"
     misc_feature    6499..6644
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33B cps gene fragment"
     misc_feature    6645..6791
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33C cps gene fragment"
     misc_feature    6792..6872
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33D cps gene fragment"
     misc_feature    6873..7016
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 34 cps gene fragment"
     misc_feature    7017..7124
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35AC/42 cps gene fragment"
     misc_feature    7125..7248
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35B cps gene fragment"
     misc_feature    7249..7393
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35F/47F cps gene fragment"
     misc_feature    7394..7541
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 36 cps gene fragment"
     misc_feature    7542..7619
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 38 cps gene fragment"
     misc_feature    7620..7762
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 39 cps gene fragment"
     misc_feature    7763..7876
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 41A cps gene fragment"
     misc_feature    7877..8010
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 41F cps gene fragment"
     misc_feature    8011..8160
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 43 cps gene fragment"
     misc_feature    8161..8248
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 45 cps gene fragment"
     misc_feature    8249..8381
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 46 cps gene fragment"
     misc_feature    8382..8502
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 47AF cps gene fragment"
     misc_feature    8503..8635
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 48 cps gene fragment"
     primer_bind     complement(8652..8668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8676..8692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8700..8730)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8745..8766)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9054..9642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9816..10673)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10674..10778)
                     /label=AmpR promoter

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