pNTAP-TSR vector (V004267)

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LOCUS       40924_33567        3968 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTAP-TSR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Human cytomegalovirus protein kinase UL97 forms a complex with the 
            tegument phosphoprotein pp65
  JOURNAL   J. Virol. 81 (19), 10659-10668 (2007)
  PUBMED    17634236
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3968)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "19"; pages: "10659-10668"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUL-2007) Biological Chemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..234
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    235..262
                     /note="partial IgG binding domain 2; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     misc_feature    263..280
                     /label=I-SceI homing endonuclease recognition site
                     /note="I-SceI homing endonuclease recognition site"
     CDS             404..1216
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1251..1272
                     /note="partial IgG binding domain 1; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     CDS             1273..1446
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    1462..1482
                     /label=spacer region
                     /note="spacer region"
     CDS             1483..1503
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1504..1509
                     /label=spacer region
                     /note="spacer region"
     misc_feature    1510..1545
                     /note="protein C epitope; binds anti-protein C monoclonal 
                     antibody (Roche)"
     promoter        complement(1606..1624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1882..2470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2644..3501)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3502..3606)
                     /label=AmpR promoter
     promoter        3952..3968
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"