pNR.CFH vector (V004280)

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LOCUS       40924_33492        8102 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNR.CFH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Jackson HO, Bibby TS, Moore MC, Terry MJ.
  TITLE     Characterization of constitutive and inducible promoters in 
            Nannochloropsis gaditana (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Jackson HO, Bibby TS, Moore MC, Terry MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) Ocean and Earth Sciences, National 
            Oceanography Centre, Southampton, European Way, Southampton SO14 
            3ZH, England
REFERENCE   3  (bases 1 to 8102)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8102)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2017) Ocean and Earth Sciences, National Oceanography 
            Centre, Southampton, European Way, Southampton SO14 3ZH, England"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1319
                     /label=NR promoter
                     /note="NR promoter"
                     /regulatory_class="promoter"
     misc_feature    1323..1330
                     /label=NotI restriction site
                     /note="NotI restriction site"
     misc_feature    1342..1349
                     /label=AscI restriction site
                     /note="AscI restriction site"
     CDS             1356..1379
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             1386..1409
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1419..1445
                     /label=9xHis
                     /note="9xHis affinity tag"
     regulatory      1452..1959
                     /label=ATPa terminator
                     /note="ATPa terminator"
                     /regulatory_class="terminator"
     regulatory      1990..2682
                     /label=UEP promoter
                     /note="UEP promoter"
                     /regulatory_class="promoter"
     CDS             2689..3708
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      3743..3995
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4029..4045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4053..4069)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4077..4107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4122..4143)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      4184..4772
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      4878..5860
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6007..6807)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(6808..7028)
                     /label=URA3 promoter
     CDS             complement(7150..7941)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"