pNOV077 vector (V004290)

LOCUS       40924_33447        8181 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNOV077, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Jones AK, Woods AL, Takeoka KT, Shen X, Wei J-R., Caughlan RE, Dean 
            CR.
  TITLE     Determinants of antibacterial spectrum and resistance potential of 
            the elongation factor G inhibitor argyrin B in key Gram negative 
            pathogens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron Way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 8181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2016) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron Way, Emeryville, CA 94608, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1337
     CDS             complement(1474..2553)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2554..2631)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        2861..2889
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    2897..2913
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2936..5056
                     /codon_start=1
                     /gene="fusA"
                     /product="elongation factor G"
                     /label=fusA
                     /note="L663Q"
                     /protein_id="APS85745.1"
                     /translation="MARTTPINRYRNIGICAHVDAGKTTTTERVLFYTGVNHKLGEVHD
                     GAATTDWMVQEQERGITITSAAVTTFWKGSRGQYDNYRVNVIDTPGHVDFTIEVERSLR
                     VLDGAVVVFCGTSGVEPQSETVWRQANKYGVPRIVYVNKMDRQGANFLRVVEQIKKRLG
                     HTPVPVQLAIGAEENFVGQVDLIKMKAIYWNDDDKGMTYREEEIPAELKDLAEEWRSSM
                     VEAAAEANEELMNKYLEEGELSEAEIKEGLRLRTLACEIVPAVCGSSFKNKGVPLVLDA
                     VIDYLPAPTEIPAIKGVSPDDETVEDERHADDNEPFSSLAFKIATDPFVGTLTFARVYS
                     GVLSSGDSVLNSVKGKKERVGRMVQMHANQREEIKEVRAGDIAALIGMKDVTTGDTLCS
                     IEKPIILERMDFPEPVISVAVEPKTKADQEKMGIALGKLAQEDPSFRVKTDEESGQTII
                     SGMGELHLDIIVDRMKREFGVEANIGKPQVAYRETITKDNVEIEGKFVRQSGGRGQFGH
                     CWIRFSAADVDEKGNITEGLVFENEVVGGVVPKEYIPAIQKGIEEQMKNGVVAGYPLIG
                     LKATVFDGSYHDVDSNEMAFKIAASMATKQLAQKGGGKVLEPIMKVEVVTPEDYMGDVM
                     GDLNRRRGLIQGMEDTVSGKVIRAEVPQGEMFGYATDVRSMSQGRASYSMEFSKYAEAP
                     SNIVEALVKKQG"
     gene            2936..5056
                     /gene="fusA"
                     /label=fusA
                     /note="L663Q"
     primer_bind     complement(5066..5082)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5342..5428
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5520..5547
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        5579..5607
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5796..6326
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     rep_origin      6351..6806
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      6917..7505
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7691..7831)
                     /label=bom
                     /note="basis of mobility region from pBR322"