pNOV016 vector (V004295)

Download GeneBank File(.gb)

LOCUS       V004295                 5845 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004295
VERSION     V004295
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2017) Infectious Diseases, Bacteriology, Novartis
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA
            94608, USA
REFERENCE   2  (bases 1 to 5845)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5845)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (15-SEP-2017) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(38..1117)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(1118..1195)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1425..1453
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1461..1477
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1511..3202
                     /gene="COASY"
                     /label="Bifunctional coenzyme A synthase"
                     /note="Bifunctional coenzyme A synthase from Homo sapiens.
                     Accession#: Q13057"
     promoter        3243..3271
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             3460..3990
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     rep_origin      4015..4470
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      4581..5169
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5355..5495)
                     /label="bom"
                     /note="basis of mobility region from pBR322"