pNL-DT5R vector (V004300)

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LOCUS       V004300                12076 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004300
VERSION     V004300
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Kamada K, Igarashi T, Martin MA, Khamsri B, Hatcho K, Yamashita T,
            Fujita M, Uchiyama T, Adachi A.
  TITLE     Generation of HIV-1 derivatives that productively infect macaque
            monkey lymphoid cells
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 103 (45), 16959-16964 (2006)
   PUBMED   17065315
REFERENCE   2  (bases 1 to 12076)
  AUTHORS   Kamada K, Adachi A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2006) Contact:Kazuya Kamada Kobe university school
            of medicine, Department of microbiology; 7-5-1, Kusunokicho,
            Chuo-ku, Kobe, Hyogo 650-0017, Japan
REFERENCE   3  (bases 1 to 12076)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12076)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2006"; volume: "103"; issue: "45"; pages:
            "16959-16964"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JUL-2006) Contact:Kazuya Kamada Kobe university school of
            medicine, Department of microbiology; 7-5-1, Kusunokicho, Chuo-ku,
            Kobe, Hyogo 650-0017, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     CDS             790..2286
                     /codon_start=1
                     /product="gag polyprotein"
                     /label="gag polyprotein"
                     /note="Cyclophilin binding loop (PVHAGPIAP) in capsid
                     region was replaced to SIVmac analogues (PQPAPQQ)"
                     /protein_id="BAF34641.1"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPQPAPQ
                     QGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSIL
                     DIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPGAT
                     LEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEGHI
                     AKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPE
                     ESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2079..5087
                     /label="HIV-1 pol"
                     /note="pol protein from human immunodeficiency virus 1"
     misc_feature    5092..5096
                     /note="artificially inserted sequence for generating Sma I
                     site"
     CDS             5097..5741
                     /codon_start=1
                     /product="vif protein"
                     /label="vif protein"
                     /note="derived from SIVmac239"
                     /protein_id="BAF34643.1"
                     /translation="MEEEKRWIAVPTWRIPERLERWHSLIKYLKYKTKDLQKVCYVPHF
                     KVGWAWWTCSRVIFPLQEGSHLEVQGYWHLTPEKGWLSTYAVRITWYSKNFWTDVTPNY
                     ADILLHSTYFPCFTAGEVRRAIRGEQLLSCCRFPRAHKYQVPSLQYLALKVVSDVRSQG
                     ENPTWKQWRRDNRRGLRMAKQNSRGDKQRGGKPPTKGANFPGLAKVLGILA"
     CDS             5744..6031
                     /gene="vpr"
                     /label="Protein Vpr"
                     /note="Protein Vpr from Human immunodeficiency virus type 1
                     group M subtype B (isolate NY5). Accession#: P12520"
     CDS             6015..6188
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH5). Accession#: P04612"
                     /label="Protein Tat"
     CDS             6406..8964
                     /gene="env"
                     /label="Envelope glycoprotein gp160"
                     /note="Envelope glycoprotein gp160 from Human
                     immunodeficiency virus type 1 group M subtype B (isolate
                     MFA). Accession#: P19551"
     CDS             8972..8998
                     /gene="nef"
                     /label="Protein Nef"
                     /note="Protein Nef from Human immunodeficiency virus type 1
                     group M subtype D (isolate Z84). Accession#: P12481"
     LTR             9261..9894
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9919..9935)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    9943..9959
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9967..9997)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10012..10033)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10321..10909)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11083..11940)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11941..12045)
                     /label="AmpR promoter"