pNK071 vector (V004303)

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LOCUS       40924_33317        6352 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNK071, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Kandul NP, Guo M, Hay BA.
  TITLE     A positive readout single transcript reporter for site-specific mRNA
            cleavage
  JOURNAL   PeerJ (2017) In press
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Kandul NP, Guo M, Hay BA.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JAN-2017) Biology and Biological Engineering, 
            California Institute of Technology, 1200 E. California Blvd., 
            Pasadena, CA 91125, USA
REFERENCE   3  (bases 1 to 6352)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6352)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JAN-2017) Biology and Biological Engineering, California 
            Institute of Technology, 1200 E. California Blvd., Pasadena, CA 
            91125, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2606..3301
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     intron          3319..3598
     ncRNA           3337..3548
                     /label=miRNA
                     /note="miR-6.1 backbone with a synthetic guide miRNA"
                     /ncRNA_class="miRNA"
     ncRNA           3458..3479
                     /label=other
                     /note="synthetic guide miRNA"
                     /ncRNA_class="other"
     CDS             3637..3678
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             3688..3705
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(3778..3912)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4534..5122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5296..6153)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6154..6258)
                     /label=AmpR promoter