pNG168 vector (V004334)

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LOCUS       V004334                 8960 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004334
VERSION     V004334
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   DasSarma S.
  TITLE     Natural plasmids and plasmid vectors of halophiles
  JOURNAL   (in) DasSarma,S., Fleischmann,E.M., Robb,F.T., Place,A.R.,
            Sowers,K.R. and Schreier,H.J. (Eds.); ARCHAEA, A LABORATORY MANUAL -
            HALOPHILES: 241-250; Cold Spring Harbor Laboratory Press, Cold
            Spring Harbor, NY (1995)
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   DasSarma S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2003) Center of Marine Biotechnology, University
            of Maryland Biotechnology Institute, 701 E. Pratt Street, Suite 236,
            Baltimore, MD 21202
REFERENCE   3  (bases 1 to 8960)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8960)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in)
            DasSarma,S., Fleischmann,E.M., Robb,F.T., Place,A.R., Sowers,K.R.
            and Schreier,H.J. (Eds.)"; volume: " ARCHAEA, A LABORATORY MANUAL -
            HALOPHILES"; pages: " 241-250; Cold Spring Harbor Laboratory Press,
            Cold Spring Harbor, NY (1995"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2003) Center of Marine Biotechnology, University of Maryland
            Biotechnology Institute, 701 E. Pratt Street, Suite 236, Baltimore,
            MD 21202"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             849..3878
                     /codon_start=1
                     /gene="repH"
                     /product="RepH"
                     /label="repH"
                     /note="replication protein; necessary for replication in
                     haloarchaea"
                     /protein_id="AAP44985.1"
                     /translation="MHTPNQQQGIRKIVPGGTLSTAGITITEVTPRVTEWIPDLLEELL
                     PRSIQSVRKFIRQEDPEVLTHARYNTVYRRLQEETLRFDHQEWCSTTDIWSDAEAEAVE
                     YVESLVEFAVKYSDVDEDDLDELSEYHQQRCKSLKQTLTTISTGRGPLNAGLEALAKGP
                     VRLHDELDDAPQPITLVLDGELWSKLDDRGTGIRALAAIAVLGSTFDVRLVISPALDAA
                     IERRYPDWYDSHLRLTETRETSSVESAGGDGQPSAEQLEEAWEAIQNLPEESGRLRLLR
                     NLPIEGSRDYRDLKQDDEIDVQAGTVGRYILDLEELGLVDIDRRGQYNSASLTGLGQVA
                     VEQYVTTDYRVIHPTQSTLETHLTPTPQPQASTVYPARSDTREGDQPGTAEDWIAATGS
                     PSEGADYVQWLDGPSGVLDAWGMHQRYLAGRRDRGVTLVDDRIERFEDGRVSYLSCFDD
                     DLFVATQWGGPLPTLGRIAGALLSDKALSKILTPSRLGNQFEEINDAVVEQLDREAGEI
                     IRRGHQIGWFSEDEEDYDGWRERIGSVRSLCLQQVGELTNSDDVEARTELLRDLHGLVA
                     SATQLYYAAGVDVTINVRVPDTGMLISDERRLDDFLGFARYTIPKQSVYGIHSGYRMLL
                     EDRPEKLKRRLPYEVDDADSTMHLTASWVFSGSTMIDLHDDIEDAIEMETNEIREAIAN
                     GQESAPVMEIPVQIGNSYSAIRNHVEDYASAKNYQVAHQEDIHEGKQDLERLVRLFLRV
                     LGTEDRPHRACPHDVAEAMLHVAQSSRNYDFITVRDISYGLSNLPTKRLLPELPPTATK
                     LLKTLLDADDPMGRSEIIDTADISESSYDRYINELAAWDIIEPREIEGHRRWEAHLEPW
                     WTPQSDRDEPYADPDPDTGILYAEFPRDVASAVMCHLITHYDLPDLETAYLEGIQPGDD
                     IKALFDDHDRLRRWRPFLWGAFADSDKLERGPSGTAASDSTVVRLGQSPGPDTAQSSFQ
                     DVSETATQRDRLSQPSPGLD"
     gene            849..3878
                     /gene="repH"
                     /label="repH"
     protein_bind    4431..4452
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4467..4497
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4505..4521
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4529..4545
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4566..4584
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(4597..4704)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(4713..4731)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4741..4757)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4899..5354
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5380..5484
                     /label="AmpR promoter"
     CDS             5485..6342
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6516..7104
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7428..8636)
                     /gene="hmgA"
                     /label="3-hydroxy-3-methylglutaryl-coenzyme A reductase"
                     /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase from
                     Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879
                     / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2). Accession#:
                     Q59468"