pNDC_SUB4 vector (V004338)

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LOCUS       40924_33118        9246 bp DNA     circular SYN 14-DEC-2018
DEFINITION  Expression vector pNDC_SUB4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Whole gene expression profiles of dermatophytes during infection and
            their virulance traits
  JOURNAL   Thesis (2015) Centre Hospitalier Universitaire Vaudois
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2015) Department of Dermatology, Centre 
            Hospitalier de Lausanne (CHUV), Bugnon 48, 1011 Lausanne, 
            SWITZERLAND
REFERENCE   3  (bases 1 to 9246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Thesis 
            (2015) Centre Hospitalier Universitaire Vaudois"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2015) Department of Dermatology, Centre Hospitalier de 
            Lausanne (CHUV), Bugnon 48, 1011 Lausanne, SWITZERLAND"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             273..1064
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1366..2511
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    2640..2664
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2698..2722
                     /note="LB T-DNA repeat; left border repeat from nopaline
                     C58 T-DNA"
     primer_bind     2958..2974
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3846..4868)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     CDS             complement(6263..7486)
                     /codon_start=1
                     /product="subtilisin-like protease SUB4"
                     /label=subtilisin-like protease SUB4
                     /note="sub4"
                     /protein_id="CRN13537.1"
                     /translation="MVCLKTLSVFLAAFAAADARAVFKTQGHKNSEMIPDNYIVVMKDG
                     VSQDDFKAHISSVASIHSTNKAKRGTNTQGMKREFDIMNWRGYHGHFDRDTLEEILNDS
                     KVDYVEQDQVVRISGLVTQRSAPSWGLGRVSHRQAGSRDYVFDDSAGRGVTIYGVDTGI
                     DINHQDFRGRARWGTNTADRDNADRHGHGTHTASTFAGTAYGIAKNANIVAVKVLGSDG
                     SGSTSGIIAGINYCVQDAQQRGILGKAAMNLSLGGGFSQANNDAVTRAQNAGIFVAVAA
                     GNDNRDARNYSPASAPAVCTVASSTINDSKSSFSNWGPVELKANITSVDIYAPGSDIIA
                     ARPGGGSTTMSGTSMASPHVAGMGAYMIGMGADPRQVCDRLKQLATAAIRNPGSSTTNR
                     LLYNGSGQ"
     misc_feature    8443..8467
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8606..9237
                     /label=oriV
                     /note="incP origin of replication"