pND50 vector (V004341)

LOCUS       V004341                 3772 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004341
VERSION     V004341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Yamagishi J, Kojima T, Oyamada Y, Fujimoto K, Hattori H, Nakamura S,
            Inoue M.
  TITLE     Alterations in the DNA topoisomerase IV grlA gene responsible for
            quinolone resistance in Staphylococcus aureus
  JOURNAL   Antimicrob. Agents Chemother. 40 (5), 1157-1163 (1996)
   PUBMED   8723458
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Inoue R, Kaito C, Tanabe M, Kamura K, Akimitsu N, Sekimizu K.
  TITLE     Genetic identification of two distinct DNA polymerases, DnaE and
            PolC, that are essential for chromosomal DNA replication in
            Staphylococcus aureus
  JOURNAL   Mol. Genet. Genomics 266 (4), 564-571 (2001)
   PUBMED   11810227
REFERENCE   3  (bases 1 to 3772)
  AUTHORS   Matsuo M, Kurokawa K, Nishida S, Li Y, Takimura H, Kaito C, Fukuhara
            N, Maki H, Miura K, Murakami K, Sekimizu K.
  TITLE     Isolation and mutation site determination of the
            temperature-sensitive murB mutants of Staphylococcus aureus
  JOURNAL   FEMS Microbiol. Lett. 222 (1), 107-113 (2003)
   PUBMED   12757953
REFERENCE   4  (bases 1 to 3772)
  AUTHORS   Kurokawa K, Matsuo M, Sekimizu K.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2005) Kenji Kurokawa, The University of Tokyo,
            Graduate School of Pharmaceutical Sciences; 7-3-1, Hongo, Bunkyo-ku,
            Tokyo 113-0033, Japan (E-mail:kurokawa@mol.f.u-tokyo.ac.jp,
            URL:http://www.f.u-tokyo.ac.jp/~hassei/, Tel:81-3-5841-4821,
            Fax:81-3-5684-2973)
REFERENCE   5  (bases 1 to 3772)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 3772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
            Agents Chemother."; date: "1996"; volume: "40"; issue: "5"; pages:
            "1157-1163"
            SGRef: number: 2; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2001"; volume: "266"; issue: "4"; pages: "564-571"
            SGRef: number: 3; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "2003"; volume: "222"; issue: "1"; pages:
            "107-113"
            SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
            (03-MAR-2005) Kenji Kurokawa, The University of Tokyo, Graduate
            School of Pharmaceutical Sciences"; volume: " 7-3-1, Hongo,
            Bunkyo-ku, Tokyo 113-0033, Japan
            (E-mail:kurokawa@mol.f.u-tokyo.ac.jp,
            URL:http://www.f.u-tokyo.ac.jp/~hassei/, Tel:81-3-5841-4821, Fax";
            pages: "81-3-5684-2973"
            SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     rep_origin      264..852
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1430..2077)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2359..3360)
                     /label="repB"
                     /note="RepB replication protein"