pNCO-HISACT-(C49S)-ASLOV2-syn vector (V004345)

LOCUS       V004345                 4259 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004345
VERSION     V004345
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W,
            Muller F, Bacher A, Fischer M.
  TITLE     (13)C Isotopologue editing of FMN bound to phototropin domains
  JOURNAL   FEBS J. 274 (22), 5876-5890 (2007)
   PUBMED   17944933
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Fischer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2007) Chemistry, University of Hamburg,
            Grindelallee 117, Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 4259)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4259)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J.";
            date: "2007"; volume: "274"; issue: "22"; pages: "5876-5890"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2007) Chemistry, University of Hamburg, Grindelallee 117,
            Hamburg 20146, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             115..468
                     /gene="hatA"
                     /label="Hisactophilin-1"
                     /note="Hisactophilin-1 from Dictyostelium discoideum.
                     Accession#: P13231"
     CDS             487..504
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    505..978
                     /note="Region: Avena sativa phototropin PHY3 fusion protein
                     Lov2 domain"
     terminator      1001..1095
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1867..1953
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    2109..2249
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2435..3023)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3197..4054)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4055..4159)
                     /label="AmpR promoter"