pNBioSec vector (V004349)

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LOCUS       V004349                 7032 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004349
VERSION     V004349
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Mize GJ, Harris JE, Takayama TK, Kulman JD.
  TITLE     Regulated expression of active biotinylated G-protein coupled
            receptors in mammalian cells
  JOURNAL   Protein Expr. Purif. 57 (2), 280-289 (2008)
   PUBMED   18042400
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Mize GJ, Harris JE, Takayama TK, Kulman JD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2007) Department of Biochemistry, University of
            Washington, N.E. Pacific Street, Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 7032)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7032)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein
            Expr. Purif."; date: "2008"; volume: "57"; issue: "2"; pages:
            "280-289"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-AUG-2007) Department of Biochemistry, University of Washington,
            N.E. Pacific Street, Seattle, WA 98195, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..285
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        318..356
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     regulatory      477..485
                     /label="Kozak site"
                     /note="Kozak site"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    570..608
                     /label="biotin acceptor peptide (BioTag)-encoding"
                     /note="biotin acceptor peptide (BioTag)-encoding"
     misc_feature    609..628
                     /note="multiple cloning site; MCS"
     misc_feature    729..1315
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     regulatory      1326..1334
                     /label="Kozak site"
                     /note="Kozak site"
                     /regulatory_class="ribosome_binding_site"
     CDS             1335..1424
                     /label="prolactin signal sequence"
                     /note="signal sequence from bovine prolactin"
     CDS             1425..1448
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1455..2417
                     /label="BirA"
                     /note="E. coli biotin protein ligase"
     intron          2485..3057
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             3061..3183
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    3254..3309
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(3871..4459)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4633..5490)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5491..5595)
                     /label="AmpR promoter"
     promoter        5700..6029
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6122..6718
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    6951..7032
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"