Basic Vector Information
- Vector Name:
- pN-TAPa
- Antibiotic Resistance:
- Streptomycin
- Length:
- 12537 bp
- Type:
- N-terminal TAPa T-DNA vector
- Replication origin:
- ori
- Source/Author:
- Rubio V, Shen Y, Saijo Y, Liu Y, Gusmaroli G, Dinesh-Kumar SP, Deng XW.
- Promoter:
- CaMV35S(enhanced)
pN-TAPa vector Map
pN-TAPa vector Sequence
LOCUS 40924_32968 12537 bp DNA circular SYN 18-DEC-2018
DEFINITION N-terminal TAPa T-DNA vector pN-TAPa, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12537)
AUTHORS Rubio V, Shen Y, Saijo Y, Liu Y, Gusmaroli G, Dinesh-Kumar SP, Deng
XW.
TITLE An alternative tandem affinity purification strategy applied to
Arabidopsis protein complex isolation
JOURNAL Plant J. 41 (5), 767-778 (2005)
PUBMED 15703063
REFERENCE 2 (bases 1 to 12537)
AUTHORS Rubio V, Deng XW.
TITLE Direct Submission
JOURNAL Submitted (21-OCT-2004) MCDB, Yale University, 165, Prospect St.,
New Haven, CT 06511, USA
REFERENCE 3 (bases 1 to 12537)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12537)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
date: "2005"; volume: "41"; issue: "5"; pages: "767-778"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-OCT-2004) MCDB, Yale University, 165, Prospect St., New Haven,
CT 06511, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12537
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 96..769
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 791..846
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
regulatory 857..866
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 884..1057
/label=ProtA
/note="IgG-binding unit of Staphylococcus aureus protein A"
CDS 1061..1231
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/label=ProtA
/translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
EAKKLNDAQAPK"
CDS 1256..1279
/label=HRV 3C site
/note="recognition and cleavage site for human rhinovirus
3C and PreScission proteases"
CDS 1286..1303
/label=6xHis
/note="6xHis affinity tag"
CDS 1328..1357
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 1364..1393
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1400..1429
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1448..1477
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1484..1513
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1520..1549
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1568..1597
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1604..1633
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1640..1669
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
protein_bind 1704..1828
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 2002..2104
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 2105..2761
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 3106..3408
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(3452..3576)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
terminator 3605..3857
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3873..3889)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3897..3913
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3921..3951)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3966..3987)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4152..4176
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 5476..6102
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 6539..7603
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 7672..7866
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 8210..8350
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(8536..9124)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9371..10159)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
misc_feature 10687..10711
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(10809..10983)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(11021..11551)
/label=GmR
/note="gentamycin acetyltransferase"
promoter complement(11611..12289)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind 12518..12534
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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