pMWO-073 vector (V004380)

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LOCUS       40924_32833        4517 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMWO-073, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Low copy expression vectors for use in Yersinia sp. and related 
            organisms
  JOURNAL   Plasmid 68 (1), 33-42 (2012)
  PUBMED    22445322
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Microbiology and Immunology, University of 
            North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen 
            Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE   3  (bases 1 to 4517)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4517)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Microbiology and Immunology, University of North 
            Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, 
            CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel 
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /note="MWO-fw; forward sequencing primer"
     promoter        75..148
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     regulatory      156..169
                     /regulatory_class="ribosome_binding_site"
     RBS             158..169
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    177..179
                     /label=translation start site
                     /note="translation start site"
     misc_feature    complement(180..287)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(296..314)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(423..445)
                     /note="MWO-rv-1; reverse sequencing primer"
     CDS             complement(764..1711)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(1759..1981)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2385..2471)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2525..3145)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             complement(3365..4153)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"