pMV5 vector (V004389)

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LOCUS       V004389                10047 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004389
VERSION     V004389
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Inui M, Tsuge Y, Suzuki N, Vertes AA, Yukawa H.
  TITLE     Isolation and characterization of a native composite transposon,
            Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum
            chromosomal DNA
  JOURNAL   Appl. Environ. Microbiol. 71 (1), 407-416 (2005)
   PUBMED   15640215
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2004) Masayuki Inui, Research Institute of
            Innovative Technology for the Earth (RITE), Microbiology Research
            Group; 9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan
            (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax:81-774-75-2321)
REFERENCE   3  (bases 1 to 10047)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10047)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2005"; volume: "71"; issue: "1"; pages:
            "407-416"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2004) Masayuki Inui, Research Institute of Innovative
            Technology for the Earth (RITE), Microbiology Research Group";
            volume: " 9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan
            (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax"; pages:
            "81-774-75-2321"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             870..2357
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /protein_id="BAD83816.1"
                     /translation="MYMIIPNSTPAYPISGSRVNTPSRLRPDCAGLDDTPASTVDRDKL
                     LAHLGRTALHGSISRNFKGAYRLVVDKETGEKRSVPNLYRIDSEKLGRCEYVMLTSKQY
                     ASVMVIDVDQIGEAGGHPENLNSYVKGVIWVLVQHGIGPAWAGINPISGKAQFIWLIDP
                     VYAGKNRASRNMELLKATSHELGELLDHDPHFAHRFSRSPFYTGKSPEAYRWYCQHDRV
                     IRLQDFLRQVREMAGQSQHIKNKRQQFNSGRELINAVKTRREEAQAFKALAEDVENEIS
                     EEIDQYDPELIDGVRVRWISQGVAARDETAFSHALKIGHRLRKAGQRLTDAAVIDAYEH
                     AYNVAQQQGSAGRDSEMPPMRDRQTMARRVRGYVTQSKTNTSLGASAPGRVTSSERKAL
                     ATMGRKGGQKAAQRWKTDPEGQYAQNQLQKLKKTHRKKRVEGQTTRAKIQALIGEAYVQ
                     TGEVLTRKQIVDETGLSRATVTRHLAALREQGALPET"
     gene            870..2357
                     /gene="repA"
                     /label="repA"
     regulatory      2067..2076
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4213..4229)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    4237..4253
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4261..4291)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4306..4327)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4615..5203)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5692..5708
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5749..6194
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     CDS             6195..7613
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     CDS             7823..8602
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     mobile_element  complement(8764..9531)
                     /label="IS1"
                     /note="prokaryotic transposable element"
     primer_bind     10005..10021
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"