pMV361-Edim6 vector (V004391)

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LOCUS       V004391                 5641 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004391
VERSION     V004391
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2611..3723
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     regulatory      3899..4291
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     regulatory      4256..4262
                     /label="derived from Mycobacterium bovis hsp60 RBS"
                     /note="derived from Mycobacterium bovis hsp60 RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             4274..5503
                     /codon_start=1
                     /product="Hsp60/VP6 chimera"
                     /label="Hsp60/VP6 chimera"
                     /note="first six amino acids of Mycobacterium bovis hsp60
                     linked to 397 amino acids of EDIM rotavirus VP6"
                     /protein_id="AAZ16497.1"
                     /translation="MAKTIADPAAEFMDVLYSISRTLKDARDKIVEGTLYSNVSDLIQQ
                     FNQMLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLNLDANYVESARTTIDYFVDFI
                     DNVCMDEMMRESQRNGIAPQSDALRKLSGVKFRRINFNNSSEYIENWNLQNRRQRTGFT
                     FHKPNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQVAGFDYSCAINAPANIQQFEH
                     IVQLRRVLTTATITLLPDAERFSFPRVINSADGATTWYFNPVILRPNNVEVEFLLNGQV
                     INTYQARFGTIVARNFDTIRLSFQLMRPPNMTPAVTALFPNAQPFEHHATVGLTLRIDS
                     AICESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWTDLITNYSPSREDNLQRVFT
                     VASIRSMLVK"
     misc_feature    4274..4291
                     /label="derived from Mycobacterium bovis hsp60 protein"
                     /note="derived from Mycobacterium bovis hsp60 protein"
     misc_feature    4310..5503
                     /note="derived from EDIM rotavirus VP6 (Choi et al., 1997)"
     terminator      5559..5605
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"