pMV33A-Edim6 vector (V004392)

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LOCUS       V004392                 6508 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004392
VERSION     V004392
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6508)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-JUN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2611..3723
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     regulatory      3896..4136
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     CDS             4193..5164
                     /gene="fbpB"
                     /label="Diacylglycerol acyltransferase/mycolyltransferase
                     Ag85B"
                     /note="Diacylglycerol acyltransferase/mycolyltransferase
                     Ag85B from Mycobacterium bovis (strain BCG / Pasteur
                     1173P2). Accession#: A1KJU9"
     misc_feature    5177..6370
                     /note="derived from rotavirus EDIM VP6 of GenBank Accession
                     Number U65988"
     terminator      6426..6472
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"