pMV3319-Edim6 vector (V004393)

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LOCUS       V004393                 5625 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004393
VERSION     V004393
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5625)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2611..3723
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     regulatory      3896..4136
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     CDS             4201..5487
                     /codon_start=1
                     /product="19kDa antigen/VP6 chimera"
                     /label="19kDa antigen/VP6 chimera"
                     /note="21 amino acids of Mycobacterium tuberculosis 19 kDa
                     signal sequence, first 7 amino acids of 19 kDa
                     mature-protein, 3 additional amino acids and 397 amino
                     acids of rotavirus EDIM VP6"
                     /protein_id="AAZ16495.1"
                     /translation="MKRGLTVAVAGAAILVAGLSGCSSNKSTDPKMDVLYSISRTLKDA
                     RDKIVEGTLYSNVSDLIQQFNQMLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLNL
                     DANYVESARTTIDYFVDFIDNVCMDEMMRESQRNGIAPQSDALRKLSGVKFRRINFNNS
                     SEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQVA
                     GFDYSCAINAPANIQQFEHIVQLRRVLTTATITLLPDAERFSFPRVINSADGATTWYFN
                     PVILRPNNVEVEFLLNGQVINTYQARFGTIVARNFDTIRLSFQLMRPPNMTPAVTALFP
                     NAQPFEHHATVGLTLRIDSAICESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWT
                     DLITNYSPSREDNLQRVFTVASIRSMLVK"
     misc_feature    4201..4284
                     /note="derived from Mycobacterium tuberculosis 19 kDa
                     protein of GenBank Accession Number J03838"
     sig_peptide     4201..4263
                     /note="derived from Mycobacterium tuberculosis 19 kDa
                     signal peptide"
     misc_feature    4294..5487
                     /note="derived from rotavirus EDIM VP6 of GenBank Accession
                     Number U65988"
     terminator      5543..5589
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"