pMU1810 vector (V004408)

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LOCUS       V004408                10413 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004408
VERSION     V004408
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Mearls EB, Olson DG, Herring CD, Lynd LR.
  TITLE     Development of a regulatable plasmid-based gene expression system
            for Clostridium thermocellum
  JOURNAL   Appl. Microbiol. Biotechnol. 99 (18), 7589-7599 (2015)
   PUBMED   25994254
REFERENCE   2  (bases 1 to 10413)
  AUTHORS   Argyros A, Mearls EB.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2013) Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 10413)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10413)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2015"; volume: "99"; issue: "18";
            pages: "7589-7599"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAR-2013) Engineering, Dartmouth College, 14 Engineering Dr,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(294..1094)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1095..1315)
                     /label="URA3 promoter"
     misc_feature    1343..1846
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_recomb     1888..3099
                     /label="spo0A 3' flank for homologous recombination"
                     /note="spo0A 3' flank for homologous recombination"
     misc_recomb     3100..4192
                     /label="spo0A 5' flank for homologous recombination"
                     /note="spo0A 5' flank for homologous recombination"
     CDS             complement(4311..4856)
                     /codon_start=1
                     /gene="hpt"
                     /product="Hpt"
                     /label="hpt"
                     /note="hypoxanthine phosphoribosyl transferase"
                     /protein_id="AGT95731.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            complement(4311..4856)
                     /gene="hpt"
                     /label="hpt"
     CDS             complement(4882..5529)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      complement(5530..6057)
                     /gene="gapD"
                     /regulatory_class="promoter"
     gene            complement(5530..6057)
                     /gene="gapD"
                     /label="gapD"
     misc_feature    6058..6800
                     /note="fragment of Spo0A; sporulation master regulator-like
                     protein"
     CDS             complement(6801..7379)
                     /codon_start=1
                     /gene="tdk"
                     /product="Tdk"
                     /label="tdk"
                     /note="thymidine kinase"
                     /protein_id="AGT95733.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(6801..7379)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(7380..8000)
                     /gene="cbp"
                     /regulatory_class="promoter"
     gene            complement(7380..8000)
                     /gene="cbp"
                     /label="cbp"
     CDS             complement(8110..9111)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(9803..10391)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"