pMTL-JH27 vector (V004434)

Download GeneBank File(.gb)

LOCUS       V004434                 5115 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004434
VERSION     V004434
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton
            NP.
  TITLE     Integration of DNA into bacterial chromosomes from plasmids without
            a counter-selection marker
  JOURNAL   Nucleic Acids Res. 40 (8), E59 (2012)
   PUBMED   22259038
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) School of Molecular Medical Sciences, The
            University of Nottingham, Centre for Biomolecular Sciences,
            University Park, Nottingham, Nottinghamshire NG7 2RD, United Kingdom
REFERENCE   3  (bases 1 to 5115)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5115)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2012"; volume: "40"; issue: "8"; pages: "E59"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JAN-2011) School of Molecular Medical Sciences, The University
            of Nottingham, Centre for Biomolecular Sciences, University Park,
            Nottingham, Nottinghamshire NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..308
                     /gene="pyrE fragment"
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium sporogenes NCIMB10696"
     gene            9..308
                     /gene="pyrE fragment"
                     /label="pyrE fragment"
     primer_bind     complement(427..443)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    591..1790
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium sporogenes NCIMB10696"
     CDS             809..1621
                     /codon_start=1
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /note="CBO3234-like"
                     /protein_id="AFA34979.1"
                     /translation="MAKPSIFSKDYENRMKKRKRRTFFSVIVIIVIFLGIIFTNNGIGK
                     KIKNSLNQIKEETKAEEEQKNKQQEQKENKKENQNKNAATVKKESEVPKDNNIEVQLEG
                     NIKIKLIYIEDSNKNKTIKSIDLNKNNLLYDINPSKNSIVVTNPKTQNIYLVNLNGEKQ
                     DITNKQYTSTSGTTFQKDAILASKQDYLWGVLPKFIDNDNIAYVSQLPWFNKTTKYVWM
                     YNIKNKTHLYNENISGEDIKFDKLTEKGLTVIADGKTLFLKADGSIAQ"
     RBS             1713..1721
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2169..2609
                     /codon_start=1
                     /gene="repL"
                     /product="plasmid replication protein"
                     /label="repL"
                     /protein_id="AFA34980.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     gene            2169..2609
                     /gene="repL"
                     /label="repL"
     CDS             2759..3379
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      3559..4147
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     oriT            4468..4577
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             4610..4978
                     /label="traJ"
                     /note="oriT-recognizing protein"