Basic Vector Information
- Vector Name:
- pMSW107
- Antibiotic Resistance:
- Kanamycin
- Length:
- 10933 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Wecker MS, Beaton S, Chado R, Ghirardi ML.
- Promoter:
- T3
pMSW107 vector Vector Map
pMSW107 vector Sequence
LOCUS 40924_32425 10933 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMSW107, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10933)
AUTHORS Wecker MS, Beaton S, Chado R, Ghirardi ML.
TITLE Development of a Rhodobacter capsulatus self-reporting model system
for optimizing light-dependent, [FeFe]-hydrogenase-driven H
production
JOURNAL Biotechnol. Bioeng. (2016) In press
PUBMED 27531314
REFERENCE 2 (bases 1 to 10933)
AUTHORS Wecker MS.
TITLE Direct Submission
JOURNAL Submitted (26-MAY-2016) Molecular Biology, GeneBiologics, 1047
Cherryvale Road, Boulder, CO 80303, USA
REFERENCE 3 (bases 1 to 10933)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10933)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
Bioeng. (2016) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-MAY-2016) Molecular Biology, GeneBiologics, 1047 Cherryvale
Road, Boulder, CO 80303, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10933
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 458..1249
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
protein_bind 1657..1678
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1693..1723
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1731..1747
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1755..1771
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 1792..1810
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS complement(1832..2548)
/label=EmGFP
/note="Emerald GFP"
misc_feature complement(2549..2770)
/gene="HupSL-emGFP"
/note="HupSL remnant; acts as signal sequence for the
downstream emGFP"
regulatory complement(2771..3299)
/regulatory_class="promoter"
primer_bind 3306..3322
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3356..3372)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(3405..3423)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3433..3449)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
regulatory 3881..4491
/note="fru promoter; derived from Rhodobacter capsulatus"
/regulatory_class="promoter"
CDS 4492..5544
/codon_start=1
/gene="hydE"
/product="HydE"
/label=hydE
/note="derived from Clostridium acetobutylicum strain DSM
1731; codon-optimized for Rhodobacter capsulatus"
/protein_id="AOF40442.1"
/translation="MDNIIKLINKAEVTHDLTKDELVTLLKDDTHNEEIYKAADRVREK
YVGEEVHLRGLIEFSNICKRNCMYCGLRRDNKNIKRYRLEPDEIIHLAKSAKNYGYQTV
VLQSGEDDYYTVEKMKYIVSEIKKLNMAITLSIGEKTFEEYEEYRKSGADRYLIRIETT
DKELYEKLDPKMSHENRINCLKNLRKLGYEVGSGCLVGLPNQTIESLADDILFFKEIDA
DMIGVGPFIPNEDTPLGEEKGGEFFMSVKVTALIRLLLPDINIPATTAMESLYPNGRSI
ALTSGANVVMPNVTEGEYRKLYALYPGKICVNDTPGHCRQCISLKINKINRKVSATKGF
RKKSYKESIG"
gene 4492..5544
/gene="hydE"
/label=hydE
CDS 5558..6793
/codon_start=1
/gene="hydF"
/product="HydF"
/label=hydF
/note="derived from Clostridium acetobutylicum strain DSM
1731; codon-optimized for Rhodobacter capsulatus"
/protein_id="AOF40443.1"
/translation="MNELNSTPKGERLHIALFGKTNVGKSSVINALTSQEIALVSNVKG
TTTDPVYKAMELLPLGPVMLIDTAGLDDISDLGELRRGKTLEVLSKTDVAILVFDVESG
ITEYDKNIYSLLLEKKIPLIGVLNKIDKKDYKLEDYTSQFKIPIVPISALNNKGINNLK
DELIRLAPENDDKFKIVGDLLSPGDIAVLVTPIDKAAPKGRLILPQQQTIRDILESDAI
AMVTKEFELRETLDSLRKKPKIVITDSQVFLKVAADTPKDILMTSFSILMARHKGDLIE
LARGARAIEDLKDGDKILIAEACTHHRQSDDIGKVKIPRWLRQKTGKKLEFDFSSGFSF
PPNIEDYALIVHCAGCMLNRRSMLHRIESSVKKQIPIVNYGVLIAYVQGILPRALKPFP
YADRIFNQSSRN"
gene 5558..6793
/gene="hydF"
/label=hydF
CDS 6807..8225
/codon_start=1
/gene="hydG"
/product="HydG"
/label=hydG
/note="derived from Clostridium acetobutylicum strain DSM
1731; codon-optimized for Rhodobacter capsulatus"
/protein_id="AOF40444.1"
/translation="MYNVKSKVATEFISDEEIIDSLEYAKQNKSNRELIDSIIEKAKEC
KGLTHRDAAVLLECDLEDENEKMFKLAREIKQKFYGNRIVMFAPLYLSNYCVNGCVYCP
YHHKNKHIARKKLSQEDVKRETIALQDMGHKRLALEAGEDPVNNPIEYILDCIKTIYSI
KHKNGAIRRVNVNIAATTVENYKKLKDAGIGTYILFQETYNKKSYEELHPTGPKHDYAY
HTEAMDRAMEGGIDDVGIGVLFGLNMYKYDFVGLLMHAEHLEAAMGVGPHTISVPRIRP
ADDIDPENFSNAISDEIFEKIVAIIRIAVPYTGMIVSTRESKKTRERVLELGISQISGG
SSTSVGGYVESEPEEDNSSQFEVNDNRTLDEIVNWLLEMNYIPSFCTACYREGRTGDRF
MSLVKSGQIANCCQPNALMTLKEYLEDYASSNTQKNGEALIASEVEKIPNEKVKSIVKK
HLTELKEGQRDFRF"
gene 6807..8225
/gene="hydG"
/label=hydG
CDS complement(8482..9141)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(9142..9911)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
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