Basic Vector Information
- Vector Name:
- pMQ310
- Length:
- 5461 bp
- Type:
- Allelic replacement vector
- Replication origin:
- R6K γ ori
- Host:
- Yeast
- Source/Author:
- Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ, Shanks RM.
- Promoter:
- URA3
pMQ310 vector Map
pMQ310 vector Sequence
LOCUS V004506 5461 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V004506 VERSION V004506 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 5461) AUTHORS Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ, Shanks RM. TITLE New vector tools with a hygromycin resistance marker for use with opportunistic pathogens JOURNAL Mol. Biotechnol. 48 (1), 7-14 (2011) PUBMED 20972648 REFERENCE 2 (bases 1 to 5461) AUTHORS Kalivoda EJ, Horzempa JE, Sadaf AA, Stella NA, Nau GJ, Shanks RM. TITLE Direct Submission JOURNAL Submitted (19-DEC-2009) Ophthalmology, University of Pittsburgh, EEI 1015, 203 Lothrop St, Pittsburgh, PA 15213, USA REFERENCE 3 (bases 1 to 5461) TITLE Direct Submission REFERENCE 4 (bases 1 to 5461) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biotechnol."; date: "2011"; volume: "48"; issue: "1"; pages: "7-14" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-DEC-2009) Ophthalmology, University of Pittsburgh, EEI 1015, 203 Lothrop St, Pittsburgh, PA 15213, USA" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5461 /mol_type="other DNA" /organism="synthetic DNA construct" oriT complement(352..461) /direction=LEFT /label="oriT" /note="incP origin of transfer" terminator complement(882..909) /label="rrnB T2 terminator" /note="transcription terminator T2 from the E. coli rrnB gene" terminator complement(1001..1087) /label="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1437..1453 /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants" misc_feature complement(1457..1513) /label="MCS" /note="pUC18/19 multiple cloning site" primer_bind complement(1523..1539) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1547..1563) /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1571..1601) /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind complement(1616..1637) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter complement(1688..1706) /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" rep_origin 1914..2302 /label="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS 2455..3450 /gene="hyg" /label="Hygromycin-B 7''-O-kinase" /note="Hygromycin-B 7''-O-kinase from Streptomyces hygroscopicus. Accession#: P09979" CDS complement(3862..4662) /label="URA3" /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(4663..4883) /label="URA3 promoter" misc_feature 4911..5414 /label="CEN/ARS" /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence"
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