pRS4213 vector (V003585)

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LOCUS       V003585                 8182 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003585
VERSION     V003585
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     A new family of yeast vectors and S288C-derived strains for the
            systematic analysis of gene function
  JOURNAL   Yeast 18 (6), 563-575 (2001)
   PUBMED   11284012
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Biological Sciences, University of
            Manchester, 2.205 Stopford Building, Oxford Rd, Manchester M13 9PT,
            United Kingdom
REFERENCE   3  (bases 1 to 8182)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8182)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "2001"; volume: "18"; issue: "6"; pages: "563-575"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Biological Sciences, University of Manchester, 2.205
            Stopford Building, Oxford Rd, Manchester M13 9PT, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1195..1836
                     /gene="ADE8"
                     /label="Phosphoribosylglycinamide formyltransferase"
                     /note="Phosphoribosylglycinamide formyltransferase from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P04161"
     promoter        2246..2433
                     /label="HIS3 promoter"
     CDS             2434..3093
                     /label="HIS3"
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     rep_origin      complement(3811..4266)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4407..4423
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4433..4451
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(4460..4567)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(4580..4598)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4619..4635)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4643..4659)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4667..4697)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4712..4733)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5021..5609)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5783..6640)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6641..6745)
                     /label="AmpR promoter"
     rep_origin      complement(6772..8114)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"