pRS4210 vector (V003586)

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LOCUS       V003586                 7168 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003586
VERSION     V003586
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     A new family of yeast vectors and S288C-derived strains for the
            systematic analysis of gene function
  JOURNAL   Yeast 18 (6), 563-575 (2001)
   PUBMED   11284012
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Biological Sciences, University of
            Manchester, 2.205 Stopford Building, Oxford Rd, Manchester M13 9PT,
            United Kingdom
REFERENCE   3  (bases 1 to 7168)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7168)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "2001"; volume: "18"; issue: "6"; pages: "563-575"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Biological Sciences, University of Manchester, 2.205
            Stopford Building, Oxford Rd, Manchester M13 9PT, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1195..1836
                     /gene="ADE8"
                     /label="Phosphoribosylglycinamide formyltransferase"
                     /note="Phosphoribosylglycinamide formyltransferase from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P04161"
     rep_origin      complement(2797..3252)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3393..3409
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3419..3437
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(3446..3553)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3566..3584)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3605..3621)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3629..3645)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3653..3683)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3698..3719)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4007..4595)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4769..5626)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5627..5731)
                     /label="AmpR promoter"
     rep_origin      complement(5758..7100)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"