Basic Vector Information
- Vector Name:
- pRP-LB-GFP-N
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8224 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Soysa R, Tran KD, Ullman B, Yates PA.
pRP-LB-GFP-N vector Map
pRP-LB-GFP-N vector Sequence
LOCUS 40924_37543 8224 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pRP-LB-GFP-N, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8224) AUTHORS Soysa R, Tran KD, Ullman B, Yates PA. TITLE Integrating ribosomal promoter vectors that offer a choice of constitutive expression profiles in Leishmania donovani JOURNAL Mol. Biochem. Parasitol. (2016) In press PUBMED 26844641 REFERENCE 2 (bases 1 to 8224) AUTHORS Yates PA, Ullman B, Soysa R. TITLE Direct Submission JOURNAL REFERENCE 3 (bases 1 to 8224) TITLE Direct Submission REFERENCE 4 (bases 1 to 8224) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biochem. Parasitol. (2016) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-DEC-2015) Biochemistry " COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8224 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 981..1781 /label=NeoR/KanR /note="aminoglycoside phosphotransferase from Tn5" polyA_signal 1791..1839 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" CDS complement(1962..1973) /label=Factor Xa site /note="Factor Xa recognition and cleavage site" rep_origin complement(3462..4050) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4224..5081) /label=AmpR /note="beta-lactamase" promoter complement(5082..5186) /label=AmpR promoter misc_feature 5356..6003 /note="derived from Leishmania donovani ribosomal RNA spacer region" regulatory 6030..6579 /note="derived from Leishmania donovani ribosomal RNA genes" /regulatory_class="promoter" misc_feature 6601..6682 /note="contains artificial trans-splice signal and 5-UTR; leishmanial consensus splice acceptor site; LdPARP" CDS 6691..7407 /label=EGFP /note="enhanced GFP" misc_feature 7435..7527 /label=multiple cloning site /note="multiple cloning site" misc_feature 7534..8217 /label=LmSIDER 1270 /note="LmSIDER 1270"
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