pRML1 vector (V003674)

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LOCUS       40924_37408        9742 bp DNA     circular SYN 18-DEC-2018
DEFINITION  YAC construction vector pRML1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Knight TF Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 
            02139, USA
REFERENCE   2  (bases 1 to 9742)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(52..70)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(664..956)
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     promoter        complement(973..1414)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     rep_origin      complement(1548..2385)
                     /direction=LEFT
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     promoter        complement(2899..3180)
                     /label=TRP1 promoter
     promoter        3922..3984
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           4747..4786
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             4804..5931
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     polyA_signal    5976..6024
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7061..7165
                     /label=AmpR promoter
     CDS             7166..8023
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8197..8785
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9097..9723
                     /note="telomere region from pYAC4 of GenBank Accession
                     Number U01086"